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Determination of a guanosine - malonaldehyde adduct in urine by HPLC with a thiobarbituric acid reaction detector
Summary
Type:
HPLC
Application #:
107180
Catalog#:
720018.46 - MN HPLC Column, Nucleosil C18, 100A, 7um, 4.6x250mm
Detection:
fluorescence, excitation 520nm, emission 550nm
Conditions:
eluent: 7% (v/v) acetonitrile - water, flow rate 1ml/min; detection: reagent 0.5% 2-thiobarbituric acid in 10% (v/v) H3PO4 - water flow rate 0.3ml/min reaction temperature 85°C
Authors:
Seto H
Source:
J Chromatogr 570 (1991) 301 - 307
Details
Legend: Fig. 5. Chromatogram after 2-thiobarbituric acid postcolumn labeling of authentic GMA and urine extracts, A) 1.7pmol authentic GMA, B) rat urine, C) human urine. also see H. Seto, T. Seto et al.
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